Circular bioprocesses: how to transform waste into high-value bioproducts

Circular bioprocesses: how to transform waste into high-value bioproducts

Imagine if waste stopped being a problem for companies and became a source of income. This is not a futuristic idea but an increasingly tangible trend.


In a world where natural resources are finite and waste is growing exponentially, the transition towards a circular bioeconomy stands out as an essential pillar for a sustainable future—especially considering that every year, millions of tonnes of agri-industrial by-products, food waste, and organic streams remain underused, despite their high content of carbon, nutrients, and valuable compounds.


So much so that it is estimated that around 10% of food available for consumption in the EU is wasted in the supply and consumption sectors (households, food service, and retail), according to Eurostat, the Statistical Office of the European Union. But what if this waste, far from being a problem, could be turned into an opportunity—and become the raw material of the future?


Each year, around 59 million tonnes of food waste are generated in the EU, equivalent to 132 kg per person, with an estimated economic value of €132 billion (Eurostat, 2022). Behind these figures lies an opportunity for innovation: transforming this waste into bioplastics, organic acids, proteins, or biofuels capable of replacing fossil-based derivatives and reducing the industry’s carbon footprint—potentially meeting up to 20% of its demand for basic chemicals with renewable carbon.


The concept of a circular bioprocess goes beyond recycling. It involves redesigning production flows so that each carbon molecule has more than one life. As highlighted in the European Bioeconomy Strategy (2024–2025), the challenge lies in turning agricultural and urban waste into feedstocks for new bioproducts, thereby reducing impacts on soil, water, and biodiversity.

Flow of a circular bioprocess

This momentum is being reinforced by new regulations: the Packaging and Packaging Waste Regulation (PPWR), which will take general effect in August 2026 and requires all packaging to be recyclable or reusable (Design4Recycling). This regulation is creating a ripple effect throughout the value chain, where the demand for bio-based and recyclable materials is growing at an unprecedented pace.


Industrial biotechnology is today an essential tool for transforming organic waste, lignocellulosic biomass, or even CO₂ emissions into high value-added molecules. This conversion is achieved through platforms that combine microbiology, catalysis, and green chemistry. In CARTIF’s Biotechnology and Sustainable Chemistry (BQS) area, the process is structured around four main stages:

  • Smart Pretreatment: The first step is to break down the complex structure of the waste (lignocellulosic biomass, molasses, used oils) through physical, chemical, or enzymatic methods to release sugars and fermentable compounds.
  • Advanced Fermentation: At this stage, engineered microorganisms convert substrates (sugars, CO₂, syngas) into organic acids, biopolymers, alcohols, or single-cell proteins (SCP). This is a critical step, as productivity, selectivity, and stability determine the feasibility of the process.
  • Selective Biocatalysis: To convert an intermediate metabolite into a final molecule of interest, specific enzymes or biocatalytic pathways are used. These operate under mild conditions and increase the purity of the final product.
  • Separation and Purification Stage (Downstream): Membranes, chromatography, ultrafiltration, or spray drying techniques are used to isolate, concentrate, and prepare the product to meet industrial and quality regulatory requirements.

When all these processes are integrated into a biorefinery —which simultaneously produces several bioproducts from a single waste stream— carbon use is maximized, while costs, emissions, and risks associated with fossil raw materials are reduced.

In the Biotechnology area, we work with methodologies based on the development of technologies at the laboratory scale for subsequent scaling up to pilot plant and pre-industrial phases (TRL 2–5). These are accompanied by techno-economic analysis and carbon footprint assessment tools to ensure that innovation is both scalable and transferable to industry and the productive sector.


It is not enough for a process to work — it must produce competitive products in terms of volume, cost, and quality. Circular bioprocesses make it possible to access growing industrial markets. Among the bioproducts with the greatest commercial potential are:

Organic acids (lactic, acetic, succinic): building blocks for the chemical, cosmetic, and bioplastics industries.

PHA/PHB biopolymers: biodegradable alternatives with high potential in sustainable packaging.

Microbial proteins: a source of alternative protein for animal feed or aquaculture.

Natural antioxidants and bioactive peptides: high-value ingredients for nutraceuticals and cosmetics.

Bio-oils and biochars: precursors for adhesives, coatings, or porous materials.

The European market has already begun to turn interest into figures: with a high growth rate, competition among biotechnological producers is increasingly focused on niches where local supply chains, sustainability, and traceability are differentiating factors compared to fossil-based plastics.

In 2024, the packaging sector accounted for 45% of the demand for bioplastics in Europe (European Bioplastics). Forecasts point to an annual growth rate of 18% between 2025 and 2030, increasing from 0.67 to 1.54 million tonnes. Other segments, such as bioactive ingredients and technical biopolymers, are also joining this momentum, where traceability and renewable origin have become key competitive advantages.


Turning a good idea into a viable industrial project requires an advanced technological platform, flexibility, and expertise in scale-up processes. This is where CARTIF contributes the experience of its highly qualified technical staff and its comprehensive laboratory and pilot plant infrastructure.

The Biotechnology and Sustainable Chemistry (BQS) area has a complete infrastructure that enables the scaling of processes from laboratory to pilot plant, featuring automated fermenters (1–200 liters), pressurized reactors capable of using gases such as CO₂ / H₂ / CO, SCADA systems, and a state-of-the-art analytical laboratory (HPLC, GC-MS, UPLC-MS, FTIR, SEM, TGA, etc.).

With these capabilities, we can simulate industrial conditions, optimize key parameters (yields, productivity, enzymatic/energy costs), and validate feasibility before scaling up.


For those working in companies, clusters, or technology centers, this quick guide can help design a strategy to valorize and benefit from by-product and waste streams:

1. Identify your residual streams: analyze their composition, volume, and variability.

2. Define your product portfolio: select one or two “anchor products” plus potential co-products.

3. Choose a technology and develop it with innovation and competitiveness criteria — from laboratory to pilot scale — with clear KPIs such as productivity, titers, and gross/net yield.

4. Conduct economic (TEA) and environmental (LCA) assessments under relevant regulatory scenarios.

5. Secure supply and off-take agreements with suppliers and distributors.

Thanks to its multidisciplinary expertise and collaborative network with companies, CARTIF supports industry throughout the entire development cycle — from waste characterization to pilot validation and techno-economic evaluation — applying an integrated approach that reduces technological risk and accelerates the transfer of results to the market.

In summary, biotechnological waste valorization is no longer a futuristic promise: it has become a necessary strategy for companies seeking to stay ahead of regulations, reduce costs or environmental reputation risks, and capture new market niches. With strict regulations such as the PPWR coming into force and ambitious targets set for 2030, those who integrate circular bioprocesses will gain a solid competitive advantage.

Circular bioprocesses offer a real pathway to transform environmental challenges into opportunities for innovation. At CARTIF — and specifically within the BQS area — we work to ensure that every molecule counts, driving a more sustainable, competitive, and knowledge-based industry.

The green molecule revolution

The green molecule revolution

In the transition to a more sustainable world, green hydrogen has emerged as an essential resource to decarbonise key sectors such as industry and transport. In 2024, the European Union and other countries have redoubled their efforts with historic investments to build infrastructure and promote the production of renewable hydrogen, which will be crucial to meeting climate targets. This investment underlines the key role of green hydrogen in combating climate change and creating a carbon-free economy.

Green hydrogen, unlike conventional hydrogen, is generated from renewable energy-based technologies (e.g. from electrolytic cells combined with renewable energies such as wind or solar) without emitting polluting gases. This process makes it a clean and safe option for reducing global emissions. However, its mass adoption depends on successful transport and storage challenges, and this is where hydrogen carrier molecules play an essential role.

Hydrogen in its pure state is difficult to store and transport because of its low energy density and because it requires special pressure and temperature conditions. Carrier molecules, such as methanol, ammonia and formic acid, allow hydrogen to be stored safely and stably, making it easier to handle and transport. These molecules act as ‘packaging’ for the hydrogen, which can be released at the point of consumption without logistical complications.

Methanol, a versatile carrier, is obtained by combining green hydrogen with captured CO₂, and can be practically reconverted to hydrogen at the point of use. Ammonia is another promising carrier, with a high hydrogen density and an existing transport infrastructure, making it ideal for large-scale industrial applications. The lesser-known formic acid is easy to handle and an excellent choice for smaller applications, such as fuel cells in light-duty vehicles.

The flexibility of these carrier molecules opens up a wide range of applications. In the transport sector, they can be used in trucks, trains and buses, enabling carbon-free mobility. This year we have seen the first hydrogen buses operating in Germany, and Japan has launched hydrogen trains, showing the potential of hydrogen in sustainable public transport. Carrier molecules make storing and refuelling green hydrogen more practical, helping to reduce dependence on fossil fuels over long distances.

Fuente: Freepik.es

In industry, green hydrogen and its carriers are viable alternatives to replace coal in high-temperature processes, such as steel production, and as a feedstock in the chemical industry, where green hydrogen replaces grey hydrogen in the production of ammonia and methanol, essential chemicals in the manufacture of fertilisers and plastics.

In addition, green hydrogen is also key to energy storage. With the growth of renewable energies such as solar and wind, efficient methods are needed to store excess energy and release it when needed. Surplus renewable energy can be converted into green hydrogen and stored in carriers such as methanol or ammonia, which can then be converted back into energy when demand is high or renewable generation is low. This helps to make the electricity grid more stable and sustainable, and reduces the intermittency of renewable sources.

Despite its potential, green hydrogen still faces significant challenges. One of these is the cost of production, which remains high compared to fossil fuels. However, technological progress and government support are reducing these costs, with expectations that green hydrogen will become more accessible in the coming years. In addition, investments in distribution infrastructure and refuelling stations are needed to bring green hydrogen to scale, enabling its use in industrial and transport applications around the world.

CARTIF’s Biotechnology and Sustainable Chemistry Area is also developing technologies to make the production of green hydrogen more efficient and economical, reducing the costs of electrolysis and improving materials for the safe storage of hydrogen in carrier molecules. These advances bring these technologies closer to commercial scale, making green hydrogen competitive and accessible in an energy market that increasingly demands sustainability. Through projects such as CATCO2NVERS and H2METAMO, we are working on capturing CO₂ for conversion into green methanol, a high value-added hydrogen carrier. These projects not only investigate how methanol and ammonia can facilitate hydrogen storage and transport, but also explore the potential of these carriers for direct use in industrial and energy applications.

“At CARTIF, we are pioneers in green hydrogen and its chemical storage in the form of green molecules and are committed to the advancement of green hydrogen and its carriers as a solution for a low carbon economy.”

In short, green hydrogen and its derivatives are beginning to transform the way we think about energy. This resource represents a unique opportunity to reduce carbon emissions and provide clean energy in a variety of industries and applications. At CARTIF, we believe that green hydrogen is the path to a sustainable future and we are committed to developing technologies that enable its mass adoption to have a positive impact on the planet.


David Díez Rodríguez. Researcher at the Biotechnology and Sustainable Chemistry area.

Biogenic CO2: challenges and opportunities for a sustainable future

Biogenic CO2: challenges and opportunities for a sustainable future

In the fight against climate change, technological innovations is one of our most powerful allies. One of the most promising and challenging areas in this regard is the transformation of carbon dioxide (CO2), a prevalent greenhouse gas, into useful raw materials for industry and transport. This approach not only promises to mitigate greenhouse gas emissions, but also opens the door to a circular economy where waste becomes a resource.

CO2 is the main contributor to global warming, arising mainly from the burning of fossil fuels and deforestation. The concentration of CO2 in the atmosphere has unprecedented levels, making it imperative to find effective ways to reduce these emissions. Capturing and utilising of CO2 is a promising strategy, transforming this gas into valuable products, which could revolutionise sectors such as transport and manufacturing, significantly reducing our carbon footprint.

CO2 transformation into raw materials involves several methods, including electrochemistry, catalysis and biotechnology. These technologies aim to convert CO2 into fuels, plastics, building materials and other industrial chemicals, which basically fall into three types:

  1. Biotechnology: based on biological fermentation processes with gas-liquid phase substrate. It uses genetically modified organisms, such as microalgae and bacteria, to absorb CO2 and convert it into biofuels an chemicals. This approach offers the potential for highly sustainable processes that can operate under ambient conditions.
Methanol
  1. Electrochemical technology: based on the use of electrical energy and potential difference between two electrodes to reduce CO2 into value-added chemicals (e.g. methanol, formic acid, etc.) which can be used as e-fuel, H2-bearing green molecules, or chemical precursos for industrial use. The efficiency of these processes has improved significantly, but they still face challenges in terms of scalability and costs.
  1. Chemical-catalytic processes: based on the use of catalysers to active and accelerate the chemical reaction and transformation of CO2 into value-added products (methane, methanol, dimethyl ether, ,etc.)Current research lines are exploring new catalysts that can operate at low temperatures and pressures, making the process more energy efficient and economically viable.

On the other hand, CO2 transformation faces technical, economic and regulatory hurdles. Energy efficiency, cost reduction and integration of these technologies into existing infrastructure are key challenges. In addition, a regulatory framework is required to promote investment in these technologies and the use of CO2 products.

Despite these challenges, the capture and uses of CO2 as a renewable carbon source and to contribute to the decarbonisation of industry and transport, offers an unprecedented opportunity to mitigate climate change and advance towards a more sustainable and circular economy. By turning a problem into a solution, we can unlock new pathways for environmental sustainability, technological innovation and economic growth. Collaboration between governments, industries and scientific communities will be essential to overcome these challenges and harness the potential of these technologies for a greener future.

R&D projects such as CO2SMOS, coordinated by CARTIF´s Biotechnology and Sustainable Chemistry area, aims to develop a set of innovative, scalable and directly applied technologies in the bio-based industries sector that will help to convert biogenic CO2 emissions into value-added chemicals for direct use in the synthesis of low carbon footprint material bioproducts. To this end,and integrated hybrid solution is proposed that combines innovative technologies and intensified electrochemical/catalytic conversion and precision fermentation processes, together with the use of renewable vector soruces such as green H2 and biomass. Key elements to achieve the indsutry´s goal of zero-emissions and climate neutrality.